Antitumor studies

KAC prepares cancer-bearing animal models by implanting mouse/rat/human cancer cells into the subcutaneous tissue, skin, organs, brain and veins for antitumor evaluation in mice/rats. We also have experience in the provision of cancer-bearing mice.

Study steps

1. Cell culture

   2 to 3 weeks

2. Implantation

   Engraftment and proliferation of tumor (periods differ by cell)

3. Grouping, administration

   Measurement of diameter of tumors

4. Weighing of tumors

Experience

Human tumor cells (Xenograft)

Pancreatic cancer	MIA-PaCa-2
Cervical cancer	HeLa
Colon cancer	HCT-116
Glioblastoma	U87-MG

Mouse tumor cells

Orthotopic model

Implantation of human pancreatic cancer MIA-PaCa-2 cell into the subcapsular portion of pancreas in nude mice

[Study system]

BALB/c Slc-nu/nu, male, 6 weeks of age

[Implantation]

Cells: human pancreatic cancer MIA PaCa-2 cells, implantation site: subcapsular portion of the pancreas

[Anticancer drug]

Gemcitabine, twice weekly in the caudal vein

[Observation]

General condition was observed daily after the delivery and body weight was measured once a week or more after the implantation.
Samples were taken on Day 53 of implantation and tumor weight (including the pancreas) was measured.

Implantation of human glioblastoma U87-MG cells into the cerebral hemisphere striatum in NOD-SCID mice

[Study system]

NOD-SCID

[Implantation]

Cells: human glioblastoma U87-MG cells,
implantation site: cerebral hemisphere striatum

[Anticancer drug/mode of administration]

Temozolomide

[Observation/sampling]

Study of survival and proliferation of iPS cell-derived nerve cells implanted into the brain of NOD-SCID mice for evaluation of their tumorigenicity